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Merck
CN

178615

Anti-Aquaporin 5 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-AQP5

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rat, mouse, human
Application:
Citations:
6
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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

rat, mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (0.5-1.0 µg/mL)
Immunoblotting (1-10 µg/mL)
Immunofluorescence (2-20 µg/mL)
Immunohistochemistry (2-20 µg/mL))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.

Immunogen

a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5

Application

ELISA (0.5-1.0 µg/ml)

Immunoblotting (1-10 µg/ml)

Immunofluorescence (2-20 µg/ml)

Immunohistochemistry (2-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Positive Control
Epithelial cells

Other Notes

Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Devendra Kumar Maurya et al.
PloS one, 10(6), e0129171-e0129171 (2015-06-13)
TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at
Ninche Ninche et al.
Development (Cambridge, England), 147(19) (2020-10-01)
Salivary glands exert exocrine secretory function to provide saliva for lubrication and protection of the oral cavity. Its epithelium consists of several differentiated cell types, including acinar, ductal and myoepithelial cells, that are maintained in a lineage-restricted manner during homeostasis
Marta F Fidalgo et al.
Life science alliance, 5(12) (2022-10-12)
Adaptation to breathing is a critical step in lung function and it is crucial for organismal survival. Alveoli are the lung gas exchange units and their development, from late embryonic to early postnatal stages, requires feedbacks between multiple cell types.



Global Trade Item Number

SKUGTIN
178615-50UG04055977204926