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343209

Fluorescein Diacetate

≥99% (HPLC), solid, esterase substrate, Calbiochem®

Synonym(s):

Fluorescein Diacetate

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About This Item

Empirical Formula (Hill Notation):
C24H16O7
CAS Number:
596-09-8
Molecular Weight:
416.38
UNSPSC Code:
12171500
NACRES:
NA.77
MDL number:
MFCD00005062
Form:
solid
Quality level:
100
Storage condition:
OK to freeze, protect from light
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Product Name

Fluorescein Diacetate, Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

colorless to yellow

solubility

ethanol: soluble

shipped in

ambient

storage temp.

2-8°C

SMILES string

O1C3(c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C)c2c(cccc2)C1=O

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

InChI key

CHADEQDQBURGHL-UHFFFAOYSA-N

General description

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.
Substrate for lipases and esterases. Abs. Max: 498 nm.

Preparation Note

Unstable in solution. Reconstitute just prior to use.

Other Notes

Clements, K.M., et al. 2001. Osteoarthritis Cartilage9, 499.
Bedner, E., et al. 1998. Cytometry33, 1.
Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
343209-5GM07790788055868