362185
PNGase F Glycosidase
Removes all N-linked glycans, suitable for mass spectrometry, from Elizabethkingia meningosepticum
Synonym(s):
N-Glycosidase F, Elizabethkingia meningosepticum, Glycopeptidase F, PNGase F
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥20,000 units/mg protein, ≥4500 units/mL
Biological source:
bacterial (Elizabethkingia meningosepticum)
Product Name
N-Glycosidase F, Elizabethkingia meningosepticum,
biological source
bacterial (Elizabethkingia meningosepticum)
Quality Level
conjugate
(N-linked)
form
liquid
specific activity
≥20,000 units/mg protein, ≥4500 units/mL
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
General description
Native N-Glycosidase F from Elizabethkingia meningosepticum. Catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins. This enzyme will not cleave oligosaccharides containing α1,3-linked core fucose commonly found on plant glycoproteins. N-Glycosidase F will also fail to cleave if the asparagine to which the oligosaccharide is attached is not peptide-bonded to at least one amino acid residue at both the amino and carboxy termini.
Physical form
In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.
Other Notes
Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Fan, J.Q., and Lee, Y.C. 1997. J. Biol. Chem. 272, 27058.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 nmol denatured ribonuclease B per min at 37°C, pH 7.5. Cleavage is monitored by SDS-PAGE. One unit of N-Glycosidase F is equal to 1 IUB milliunit.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Still not finding the right product?
Explore all of our products under N-Glycosidase F, Elizabethkingia meningosepticum
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 362185-100U | 04055977213607 |