386040
Anti-Hsp90 Mouse mAb (AC88)
liquid, clone AC88, Calbiochem®
Synonym(s):
Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
AC88, monoclonal
Species reactivity:
-
Application:
—
Citations:
1
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AC88, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity (predicted by homology)
all
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Immunoblotting (1 µg/mL, colorimetric)
Immunoprecipitation
Immunocytochemistry )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSP90AA1(3320)
General description
Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.
Recognizes the ~90 kDa Hsp90 protein.
This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.
Immunogen
Achyla ambisexualis
Hsp90 isolated from water mold, Achyla ambisexualis
Application
Immunoblotting (1 µg/ml, colorimetric)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Immunocytochemistry (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM PBS, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells
Other Notes
Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Elevated levels of ribosomal proteins eL36 and eL42 control expression of Hsp90 in rhabdomyosarcoma.
Sarah Shaikho et al.
Translation (Austin, Tex.), 4(2), e1244395-e1244395 (2017-01-17)
Mammalian 90 kDa heat shock protein (Hsp90) is a ubiquitous molecular chaperone whose expression is selectively upregulated during stress, although the precise control mechanism of this increase is yet to be fully elucidated. We used polysome profiling to show that Hsp90α
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 386040-50UG | 04055977189896 |