574777
Anti-Synapsin I Rabbit pAb
lyophilized, Calbiochem®
Synonym(s):
Rabbit Anti-Synapsin I, Synapsin I Detection Antibody
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
human, rat, mouse, bovine
Application:
—
Citations:
6
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized
does not contain
preservative
species reactivity
human, rat, mouse, bovine
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(ELISA (1:2500-1:10,000)
Frozen Sections
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate))
isotype
IgG
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Syn1(20964)
General description
Recognizes the synapsin I protein in rat brain.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
Immunogen
Bovine
purified, brovine brain synapsin I
Application
ELISA (1:2500-1:10,000)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Frozen Sections (see application references)
Immunoblotting (1:200)
Immunocytochemistry (1:500-1:2500)
Immunoprecipitation (1 µg/200 µg lysate)
Physical form
Lyophilized from 5 mM ammonium bicarbonate.
Preparation Note
Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat brain
Rat brain
Other Notes
Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
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Kang-Yi Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(10), 2513-2524 (2007-03-09)
Collapsing response mediator protein-1 (CRMP-1) was initially identified in brain and has been implicated in plexin-dependent neuronal function. The high amino acid sequence identity among the five CRMPs has hindered determination of the functions of each individual CRMP. We generated
Alexandra Grubman et al.
Stem cell reports, 14(6), 1018-1032 (2020-05-23)
Multiple protocols have been published for generation of iMGLs from hESCs/iPSCs. To date, there are no guides to assist researchers to determine the most appropriate methodology for microglial studies. To establish a framework to facilitate future microglial studies, we first
Priya Srikanth et al.
Translational psychiatry, 8(1), 245-245 (2018-11-10)
The identification of convergent phenotypes in different models of psychiatric illness highlights robust phenotypes that are more likely to be implicated in disease pathophysiology. Here, we utilize human iPSCs harboring distinct mutations in DISC1 that have been found in families
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 574777-10UG | 04055977189735 |