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About This Item
UNSPSC Code:
41106202
NACRES:
NA.81
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Manufacturer/tradename:
Novagen®
Product Name
Rosetta Competent Cells - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Quality Level
biological source
Escherichia coli
manufacturer/tradename
Novagen®
storage condition
OK to freeze
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
transformation efficiency: >2×106 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
Genotype: F-ompT hsdSB(rB- mB-) gal dcm pRARE (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Other Notes
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 70953-3 | 04055977256123 |
| 70953-4 | 04055977256130 |