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AB143

Anti-Choline Acetyltransferase Antibody

CHEMICON®, rabbit polyclonal

Synonym(s):

Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, human, feline, bat, rat, monkey
Application:
IHC (p), IP, WB
Citations:
236
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Product Name

Anti-Choline Acetyltransferase (ChAT) Antibody, serum, Chemicon®

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, feline, bat, rat, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_065574

UniProt accession no.

P28329

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

cat ... Chat(751512)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

General description

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Application

Anti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB with more than 70 product citations.
Immunoprecipitation:
1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

Optimal working dilutions and protocls must be determined by end user.

Biochem/physiol Actions

Cholinergic neurons in brain and central nervous systems.
Reactivity with other species has not been confirmed with IHC.

Physical form

Rabbit polyclonal antisera, liquid, no preservatives.

Analysis Note

Control
Cerebral cortex tissue.
Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis:
ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B M Kramer et al.
Neuroscience, 94(4), 1163-1172 (2000-01-07)
The role of the p75 nerve growth factor receptor in the retrograde transport of neurotrophins in the adult CNS was investigated by comparing the transport of 125I-labeled neurotrophins by normal and p75 nerve growth factor receptor-deficient cholinergic septohippocampal neurons. In
K Sasaki et al.
Neuroscience, 92(4), 1287-1294 (1999-07-30)
We examined the effects of repeated subcutaneous injections of an acidic fibroblast growth factor fragment analog, [Ala16] acidic fibroblast growth factor (1-29), on learning and memory and on the choline acetyltransferase immunoreactivity of forebrain neurons in senescence-accelerated mice. One group
C E E M van der Zee et al.
Neuroscience, 110(4), 641-651 (2002-04-06)
The p75 low-affinity neurotrophin receptor (p75(LNTR)) appears to have various functions that include enhancing nerve growth factor (NGF)-mediated survival by increasing TrkA (high-affinity NGF receptor) efficiency, and mediating apoptosis by acting as a ligand-regulated pro-apoptotic receptor. Here, we investigated the
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Global Trade Item Number

SKUGTIN
AB14304053252732522
AG22004053252274930