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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat
Application:
ELISA, WB
Citations:
36
Product Name
Anti-Fibronectin Antibody, Chemicon®, from rabbit
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat
packaging
antibody small pack of 25 μL
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... FN1(2335)
mouse ... Fn1(14268)
General description
262 kDa
Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Immunogen
Purified rat plasma fibronectin.
Application
Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Other species have not been tested.
The antibody reacts with the 262 kDa fibronectin protein.
Physical form
Antigen Affinity Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Western Blot: Normal rat serum
Western Blot: Normal rat serum
Routinely evaluated by Western Blot on normal rat serum.
Western Blot Analysis:
0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.
Western Blot Analysis:
0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
Welham, NV; Yamashita, M; Choi, SH; Ling, C
Testing null
Correction: cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
Welham NV, Yamashita M, Choi SH, Ling C
Testing null
null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB1954-25UL | 04054839350481 |