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Merck
CN

AB1954

Anti-Fibronectin Antibody

CHEMICON®, rabbit polyclonal

Synonym(s):

Cold-insoluble globulin, fibronectin 1, migration stimulating factor, migration-stimulating factor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat
Application:
ELISA, WB
Citations:
36
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Product Name

Anti-Fibronectin Antibody, Chemicon®, from rabbit

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, western blot: suitable

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

General description

262 kDa
Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.

Immunogen

Purified rat plasma fibronectin.

Application

Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

Other species have not been tested.
The antibody reacts with the 262 kDa fibronectin protein.

Physical form

Antigen Affinity Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot: Normal rat serum
Routinely evaluated by Western Blot on normal rat serum.

Western Blot Analysis:
0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
Welham, NV; Yamashita, M; Choi, SH; Ling, C
Testing null
Correction: cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
Welham NV, Yamashita M, Choi SH, Ling C
Testing null
null



Global Trade Item Number

SKUGTIN
AB1954-25UL04054839350481