Anti-Aquaporin 1 Antibody

28 kDa

Aquaporin 1 is an integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Epitope: Topological domain

KLH-conjugated linear peptide corresponding to the topological domain of rat Aquaporin 1.

Anti-Aquaporin 1 Antibody detects level of Aquaporin 1 & has been published & validated for use in ELISA, WB, IH.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting: 1-10 μg/mL using Chemiluminescence technique. Immunoblots reveal two bands, a lower 28 kDa band corresponding to unglycosylated form and an upper high molecular 35-60 kDa glycosylated component (Denker et al. 1988; Smith & Agre 1991) We recommend the use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Aquaporin 1 in human kidney and mouse kidney tissue.

ImmunoAffinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Aquaporin 1 in 10 µg of human kidney tissue lysate.

Replaces: AB3065

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