Anti-Orexin-2 Receptor Antibody

A 19 aa peptide near the C-terminus (cytoplasmic domain) of rat OX2R. The immunogen peptide (Catalog Number AG794) is available to perform preabsorbtion controls.

Detect Orexin-2 Receptor using this Anti-Orexin-2 Receptor Antibody validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000 against the control peptide (Catalog Number AG794).

Optimal working dilutions must be determined by end user.

Rat/Human Orexin-2. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Affinity Purified 1mg/mL solution in PBS, pH 7.4 and 0.1% BSA

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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