AB5302
Anti-Amyloid Precursor Protein (APP) Antibody
CHEMICON®, rabbit polyclonal
Synonym(s):
APP
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About This Item
Product Name
Anti-Amyloid Precursor Protein Antibody, KPI Domain, serum, Chemicon®
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, mouse, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Immunogen
Application
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Biochem/physiol Actions
Legal Information
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5302 | 04053252335037 |