Anti-Opioid Receptor µ Antibody

Synthetic peptide (Catalog number AG375) from the C-terminus of rat mu Opioid receptor corresponding to residues 384-398 of the rat mu opioid receptor.

Anti-Opioid Receptor μ Antibody-AB5509 is an antibody against Opioid Receptor for use in IC, IH.

Immunohistochemistry: 1:50-500 See Protocol.

Immunocytochemistry: 1:50-500 See Protocol.

Optimal working dilutions must be determined by the end user.

IHC Protocol: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5509. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

ICC Protocol: Mu opioid receptor transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum free media. Media was removed and cells were gently washed 3 times with serum free media. Following fixation, cells were processed for indirect immunofluorescence as described above.

Research Category
Neuroscience

Research Sub Category
Neuroinflammation & Pain

Mu Opioid receptor.

PBS containing 50% glycerol; Concentration 1.0 mg/ml

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Replaces: AB1774

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.