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Merck
CN

AB5543

Anti-MAP2 Antibody

CHEMICON®, chicken polyclonal

Synonym(s):

Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat
Application:
ICC, IHC, WB
Citations:
41
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Product Name

Anti-MAP2 Antibody, Chemicon®, from chicken

biological source

chicken

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Map2(25595)

General description

Recognizes a doublet of ~280 kDa and a doublet of ~70kDa

Immunogen

Purified bovine brain MAP2

Application

Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism
Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.

Physical form

Ammonium Sulfate Precipitation
Format: Purified
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive = cerebral cortex. Negative = liver or kidney.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

WGK 2



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shuwen Chang et al.
Nature neuroscience, 21(1), 33-40 (2017-12-13)
The synaptic vesicle (SV) protein synaptotagmin-1 (Syt1) is the Ca2+ sensor for fast synchronous release. Biochemical and structural data suggest that Syt1 interacts with phospholipids and SNARE complex, but the manner in which these interactions translate into SV fusion remains
The upregulation of specific interleukin (IL) receptor antagonists and paradoxical enhancement of neuronal apoptosis due to electrode induced strain and brain micromotion.
Karumbaiah, Lohitash, et al.
Biomaterials, 33, 5983-5996 (2012)
Cadherin expression in the developing mouse olfactory system.
Michael R Akins,Deanna L Benson,Charles A Greer
The Journal of Comparative Neurology null



Global Trade Item Number

SKUGTIN
AB554304053252403910