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ABD101

Anti-ESRRB Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Steroid hormone receptor ERR2, ERR beta-2, Estrogen receptor-like 2, Estrogen-related receptor beta, ERR-beta, Nuclear receptor subfamily 3 group B member 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.75
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, WB
Citations:
1
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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_004443

UniProt accession no.

O95718

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ESRRB(2103)

General description

The estrogen-related receptor beta (ESRRB) was initially identified in mouse embryonic carcinoma stem cells and was found to be expressed exclusively in trophoblast progenitor cells between days 6.5 and 7.5 post coitum. Recent studies have shown that ESRRB is part of an POU5F1/Oct4-centered protein interaction network in embryonic stem (ES) cells. It is thought to interact with other ES transcription factors such as NANOG within this network, activating POU5F1 transcription and thereby sustaining self-renewal and pluripotency in ES cells.
~56 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to Amino acids 376-388 of Human ESRRB protein.

Application

Immunocytochemical Analysis: 1:50 dilution of this antibody detected ESRRB in a mouse embryonic stem cells.
This Anti-ESRRB Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of ESRRB.

Analysis Note

Evaluated by Western Blot in a mouse brain E16 lysate. Western Blot Analysis: 1μg/ml of this antibody detected ESRRB in a mouse brain E16 lysate. Uncharacterized bands may be observed in some cell lysates with longer exposure times.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Daniela Cornacchia et al.
Cell stem cell, 25(1), 120-136 (2019-06-04)
Current challenges in capturing naive human pluripotent stem cells (hPSCs) suggest that the factors regulating human naive versus primed pluripotency remain incompletely defined. Here we demonstrate that the widely used Essential 8 minimal medium (E8) captures hPSCs at a naive-to-primed intermediate

Global Trade Item Number

SKUGTIN
ABD10104053252986437