Key Documents

View All Documentation

EZHIL8

Human IL-8 ELISA Kit

Name: Human IL-8 ELISA Kit
Brand: MM

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

Synonym(s):

Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor

Select a Size

Change View

About This Item

UNSPSC Code:

41116158

NACRES:

NA.84

Gene information:

human ... CXCL8(3576)

Input:

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

Species reactivity:

human

Storage temp.:

2-8°C

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Human IL-8 Conferma^® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples

Quality Level

200

species reactivity

human

parameter

50 μL sample volume (4hr assay)

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type cell culture supernatant
sample type serum

UniProt accession no.

P10145

application(s)

research use

assay range

accuracy: 77-102 (plasma)%
linearity: 105 (plasma)%, inter-assay cv: <4.7%
intra-assay cv: <4.0%
standard curve range: 0.82-200 pg/mL
accuracy: 70-105 (serum)%
linearity: 94-106 (serum)%

detection method

colorimetric (450nm/590nm)

storage temp.

2-8°C

Gene Information

human ... CXCL8(3576)

Description

General description

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:

IL-8

Research Category:

Cytokines
Inflammation & Immunology
Immune Response
Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Regulatory Information

新产品

This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

ELISA实验方法

该文章提供了4种常用的ELISA实验方法：夹心法ELISA实验方法、磷酸化检测过程、EIA检测过程以及基于细胞的检测过程

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Articles

Conferma^® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma^® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.