Skip to Content
Merck
CN

IPFL20200

Immobilon® -FL PVDF Membrane

10 sheets, 20cm x 20 cm, 0.45 µm pore size, transfer membrane with low background fluorescence

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

Immobilon®-FL PVDF Membrane, 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Quality Level

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

20 cm × 20 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane has a nominal pore size of 0.45 μm and is made up of polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. This membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biiomolecules. The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. In addition, the Immobilon®-FL membrane can be used for standard chemiluminescent or chromogenic detection. The Immobilon®-FL is recommended for optimal blotting of proteins with molecular weights greater than 20,000.

Application

Immobilon®-FL PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Membrane:
  • Designed for optimal fluorescent immunodetection
  • Extremely low background improves the sensitivity of all fluorescence detection protocols
  • suitable for all commonly used fluorescent probes at all excitation and emission wavelengths
  • compatible with standard blocking agents and buffers
  • Ideal for multiplexing and chemifluorescence detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany


Still not finding the right product?

Explore all of our products under Immobilon®-FL PVDF Membrane


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library


Articles

荧光Western blot检测因具有多重检测的方便性而变得越来越受研究者的欢迎,但这种检测仍需要专门优化的工具来获得理想的实验结果。这里我们推荐一个最佳组合,以帮助大家在荧光检测中快速获得稳定、易重复的数据。

Related Content

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

View All Related Content

Shaoqiu Jiang et al.
Cell cycle (Georgetown, Tex.), 18(11), 1241-1253 (2019-05-14)
Researches have been focusing on the role of Slit2 in angiogenesis, specifically in cell migration and vessel permeability. Nevertheless, the role of Slit2-N, the bioactive fragment of Slit2, in the proliferation of vascular endothelia in choroidal neovascularization and some related



Global Trade Item Number

SKUGTIN
IPFL2020004053252671920