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IPVH07850

Immobilon® -P PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, mini blots, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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Product Name

Immobilon®-P PVDF Membrane, 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon®-P transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is recommended for western blotting, especially for proteins greater than 20 kDa. Immobilon®-P transfer membrane can bind a wide range of molecular weight proteins. It is used for the transfer of proteins from a variety of gel matrices. When compared to a nitrocellulose membrane, the Immobilon-P PVDF membrane has a higher signal-to-noise ratio for enhanced sensitivities, improved handling characteristics and staining capabilities and increased solvent resistance.
Primary Binding Mechanisms: Electrostatic, hydrophobic;
Filter Code: IPVH

Application

Immobilon®-P PVDF Membrane has been used in western blotting.

Features and Benefits

Immobilon®-P membrane has:
  • excellent protein retention
  • high physical strength
  • broad chemical compatibility
  • suitable for a variety of staining applications and reprobing in immunodetection methods

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Rong Kang et al.
American journal of otolaryngology, 33(6), 678-684 (2012-07-10)
The purposes of this study were to determine whether autophagy was involved in cisplatin (CDDP) resistance and to investigate the role of the autophagy in the regulation of chemosensitivity to CDDP in laryngeal cancer Hep-2 cells. A WST-1 assay was
Kentaro Togashi et al.
Journal of the neurological sciences, 413, 116802-116802 (2020-04-04)
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNc). Levodopa (L-Dopa), the current main treatment for PD, reduces PD symptoms by partially replacing dopamine, but it does not slow neurodegeneration. Recent

Global Trade Item Number

SKUGTIN
IPVH0785004053252675676