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IPVH304F0

Immobilon® -P PVDF Membrane

10 sheets, 26 cm x 26 cm, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
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Product Name

Immobilon®-P PVDF Membrane, 10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Quality Level

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

26 cm × 26 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

General description

Immobilon®-P PVDF Membrane is an original, and most used, polyvinylidene fluoride (PVDF) transfer membrane for western blotting. It has a 0.45 μm pore size and is recommended for blotting proteins >20 kDa. The Immobilon®-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Application

Immobilon®-P PVDF Membrane has been used in western blotting.

Features and Benefits

  • Compatible with a variety of detection methods, including radioactive, chromogenic, and chemiluminescent techniques.
  • High protein adsorption and retention ensures greater sensitivity
  • Devoid of crack or curl like nitrocellulose. Can be cut without fracturing and reprobed multiple times.
  • Eliminates the need for blocking and reduces detection times by up to 2 hours.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
IPVH304F004053252471339