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About This Item
Product Name
Immobilon®-PSQ PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Quality Level
material
PVDF membrane, plain filter, white filter
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable, western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.2 μm pore size
capacity
262 μg/cm2 adsorption capacity (insulin), 340 μg/cm2 adsorption capacity (BSA), 448 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination
detection method
chemiluminescent, colorimetric, radioactive
shipped in
ambient
General description
Application
Features and Benefits
- 0.2 μm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Prevents blow-through of low molecular weight proteins
Legal Information
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
本页内容介绍和讨论了三种免疫印迹膜剥离和重新检测的方案。
Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
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Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| ISEQ00010 | 04053252334306 |