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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5A3.2, monoclonal
Application:
IHC, WB
Citations:
165
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
5A3.2, monoclonal
species reactivity
chicken, monkey, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... POU4F1(5457)
General description
46 kDa
Immunogen
Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein
Application
Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125
Western blot: 1:50-1:100
Optimal working dilutions must be determined by end user.
Western blot: 1:50-1:100
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis.
Badea, TC; Williams, J; Smallwood, P; Shi, M; Motajo, O; Nathans, J
The Journal of Neuroscience null
AML1/ETO proteins control POU4F1/BRN3A expression and function in t(8;21) acute myeloid leukemia.
Dunne J, Gascoyne DM, Lister TA, Brady HJ, Heidenreich O, Young BD
Cancer Research null
Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells.
Ronald S Goldstein,Oz Pomp,Irina Brokhman,Lina Ziegler
Methods in Molecular Biology null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1585 | 04053252580383 |