MAB2575
Anti-Integrin alpha 5 (ITGA5/CD49E) Antibody
CHEMICON®, rat monoclonal, BMC5
Synonym(s):
VLA-5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
BMC5, monoclonal
Species reactivity:
mouse
Application:
FACS, IHC, IP
Citations:
7
Product Name
Anti-Integrin α5β1 Antibody, clone BMC5, ascites fluid, clone BMC5, Chemicon®
biological source
rat
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BMC5, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ITGA5(3678)
Immunogen
Mouse embryonal carcinoma P19 cells.
Application
Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.
Inhibition of cell adhesion to fibronectin: 10 μL/mL
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Biochem/physiol Actions
Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Physical form
Liquid ascites.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Invasion of eukaryotic cells by Borrelia burgdorferi requires ?(1) integrins and Src kinase activity.
Wu, J; Weening, EH; Faske, JB; Hook, M; Skare, JT
Infection and Immunity null
Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.
Legate, KR; Takahashi, S; Bonakdar, N; Fabry, B; Boettiger, D; Zent, R; Fassler, R
The Embo Journal null
Jessie Zhong et al.
PloS one, 7(4), e35058-e35058 (2012-04-18)
The speed of cell migration on 2-dimensional (2D) surfaces is determined by the rate of assembly and disassembly of clustered integrin receptors known as focal adhesions. Different modes of cell migration that have been described in 3D environments are distinguished
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB2575 | 04053252315206 |