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MAB2626

Anti-Endosialin Antibody, clone B1/35

clone B1/35, from mouse

Synonym(s):

CD248 molecule, endosialin, CD164 sialomucin-like 1, endosialin, CD248 antigen, endosialin, Tumor endothelial marker 1, CD248 antigen

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
B1/35, monoclonal
Application:
FACS, IF, IP, WB
Citations:
1
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Quality Level

100

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B1/35, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_065137

UniProt accession no.

Q9HCU0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD248(57124)

General description

Endosialin is a glycoprotein on the cell surface of vascular endothelial cells in a broad range of human cancers. Endosialin is thought to be highly expressed at the points of active tissue remodeling and neovascularisation which may imply that endosialin is key in tumor growth, angiogenesis and metastasis. Endosialin has structural similarity to CD93, which involves a C-type lectin-like domain, EGF-like repeats and a glycosylated mucin-like domain. This similarity may also suggest that endosialin plays a role in innate immunity and inflammation.
~175 kDa observed

Immunogen

Diploid human fibroblasts

Application

Detect Endosialin using this Anti-Endosialin Antibody, clone B1/35 validated for use in WB, IP, IF, FC.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect Endosialin in rheumatoid fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP on AG1523 human fibroblasts (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

Immunofluorescent Analysis: A previous lot was used by an independent laboratory in IF to detect Endosialin in human rheumatoid synovium, tonsil, salivary gland and normal breast tissues (MacFadyen, J. et al. (2005). FEBS. 579(12):2569-2575).

Physical form

Format: Purified

Analysis Note

Control
AG1523 cell lysate
Evaluated by Western Blot in AG1523 cell lysate.

Western Blot Analysis: A 1:2,500 dilution of this antibody detected Endosialin in 10 µg of AG1523 cell lysate.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Satoshi Fujii et al.
Cancer medicine, 4(11), 1667-1678 (2015-09-05)
The cancer stroma, including cancer-associated fibroblasts (CAFs), is known to contribute to cancer cell progression and metastasis, suggesting that functional proteins expressed specifically in CAFs might be candidate molecular targets for cancer treatment. The purpose of the present study was

Global Trade Item Number

SKUGTIN
MAB262604053252482458