Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24

Epitope: N-terminus

Purified GABA/benzodiazepine receptor from bovine cortex.

Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.

Immunohistochemistry: 10-20 μg/mL * See protocol below.

Western blot: 20 μg/mL

Immunoprecipitation

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB339

IMMUNOHISTOCHEMISTRY

1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.

2) Sections should be incubated floating in suitable small vials.

3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.

4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).

5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C

6) Wash sections in Buffer A.

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in Buffer A.

9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat

Format: Purified

Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.