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Merck
CN

MAB3494

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

clone 4.3E8.D1, Chemicon®, from mouse

Synonym(s):

Mitochondrial Marker

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4.3E8.D1, monoclonal
Application:
ICC, IP
Citations:
10
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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4.3E8.D1, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATP5B(506)

Immunogen

Epitope: beta chain
Rat brain mitochondria

Application

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP.
Immunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Biochem/physiol Actions

Recognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proteomic identification of the involvement of the mitochondrial rieske protein in epilepsy.
Heike Junker, Kira Spate, Yalikun Suofu, Reinhard Walther, Gerold Schwarz et al.
Epilepsia null
Michael Melnick et al.
BMC developmental biology, 6, 42-42 (2006-09-09)
Human studies suggest, and mouse models clearly demonstrate, that cytomegalovirus (CMV) is dysmorphic to early organ and tissue development. CMV has a particular tropism for embryonic salivary gland and other head mesenchyme. CMV has evolved to co-opt cell signaling networks
Pamela J Yao et al.
Molecular biology of the cell, 28(3), 387-395 (2016-12-10)
Mitochondria are essential organelles whose biogenesis, structure, and function are regulated by many signaling pathways. We present evidence that, in hippocampal neurons, activation of the Sonic hedgehog (Shh) signaling pathway affects multiple aspects of mitochondria. Mitochondrial mass was increased significantly



Global Trade Item Number

SKUGTIN
MAB349404053252466731