Anti-MAP2 Antibody

Rat brain microtubule-associated protein.

Anti-MAP2A Antibody, 2B, 2C, clone HM-2 detects level of MAP2A & has been published & validated for use in WB, IH.

Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation.

Immunohistochemistry: 1:50 to 1:1000

Optimal working dilution must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Shows selective staining of dendritic trees throughout the brain in immunohistochemical staining of brain tissue. In perkinje neurons, staining is restricted to the dendrites distal to the initial dendrite segment. No staining of cell body in mature Perkinje cells but strong staining of cell bodies of developing Perkinje cells from neonatal rats. No detectable staining of axons or non-neuronal cells. Reacts specifically with all forms of MAP2 (MAP2a ~280 kDa, MAP2b ~280 kDa and MAP2c ~70 kDa) as determined by immunoblot. No cross-reactivity observed to other MAPs or tubulin.

Ascites fluid. Liquid with 15 mM sodium azide.

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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