Anti-Aggrecan Antibody

The antigen recognized by this clone, CAT-301 is a proteoglycan that is developmentaly regulated, high molecular chondroitin sulphate proteoglycan found on the extracellular surface of mammalian neurons. It is expressed late in development and although no definitive role has been identified for CAT-301, it is believed to have a role in the stabilisation of synaptic structure.

The name derives from the name of the monoclonal antibody originally used to identified by its expression, which was detected immunocytochemicaly



Disruption of the normal patterns of neuronal activity during the critical early postnatal period by physical or biochemical means results in a large and irreversible reduction in levels of CAT-301. Similar intervention in mature animals has no effect



CAT-301 is related to aggrecan from articular cartilage. The CAT-301 antibody immunoreacts with aggrecan, and the chondroitinase treated aggrecan and CAT-301 both have apparent sizes of 550kDa. However CAT- 301 is not glycosylated by keratan sulphate, as aggrecan is.

Epitope: Brain (core protein)

Feline spinal cord fixed gray matter.

Anti-Chondroitin Sulfate Proteoglycan Antibody, Brain (core protein), clone Cat-301 detects level of Chondroitin Sulfate Proteoglycan & has been published & validated for use in IP, WB, IC, IH.

Immunohistochemistry: 1:500-1:2,000 on 4% paraformaldehyde fixed frozen tissue.

Immunocytochemistry

Immunoblot

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Chondroitin sulfate proteoglycan (CSPG), brain core protein.

Format: Purified

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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