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Merck
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MAB5492

Anti-Huntingtin Disease (HD/HTT) Antibody

CHEMICON®, mouse monoclonal, 2B4

Synonym(s):

Anti-Huntingtin, Anti-Huntingtin Antibody, Detection Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
2B4, monoclonal
Species reactivity:
human
Application:
ELISA, ICC, IHC, WB
Citations:
58
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Product Name

Anti-Huntingtin Antibody, a.a. 1-82, ascites fluid, clone 2B4, Chemicon®

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2B4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

General description

348 kDa

Immunogen

Epitope: a.a. 1-82
Recombinant human huntingtin, amino acids 1-82.

Application

Anti-Huntingtin Antibody, a.a. 1-82 is an antibody against Huntingtin for use in ELISA, IC, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 1:500-1:5,000

Immunocytochemistry (1): 1:500-1:5,000

Immunohistochemistry (1,2): 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts huntingtin protein, amino acids 1-82. The antibody recognizes wild type and mutant huntingtin.

Physical form

Ascites fluid containing no preservatives.
Unpurified

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Normal human cerebral cortex lysate, Mouse brain cortex samples from HD or wild type mice

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Acetylation targets mutant huntingtin to autophagosomes for degradation.
Hyunkyung Jeong,Florian Then,Thomas J Melia,Joseph R Mazzulli,Libin Cui,Jeffrey N Savas et al.
Cell null
Xiaofeng Gu et al.
Neuron, 85(4), 726-741 (2015-02-11)
The nucleus is a critical subcellular compartment for the pathogenesis of polyglutamine disorders, including Huntington's disease (HD). Recent studies suggest the first 17-amino-acid domain (N17) of mutant huntingtin (mHTT) mediates its nuclear exclusion in cultured cells. Here, we test whether
Inducing huntingtin inclusion formation in primary neuronal cell culture and in vivo by high-capacity adenoviral vectors expressing truncated and full-length huntingtin with polyglutamine expansion.
Bin Huang, Johannes Schiefer, Christian Sass, Christoph M Kosinski, Stefan Kochanek
The journal of gene medicine null



Global Trade Item Number

SKUGTIN
MAB549204053252576782