Anti-Poly ADP-ribose Antibody, clone 10H

Observed molecular weight is the result of poly(ADP-ribose) polymer on protein receptors (such as histones and transcription factors).

Poly(ADP-ribose) polymerase is an abundant nuclear enzyme that catalyses the synthesis of poly(ADP-ribose) from nicotinamide adenine dinucleotide (NAD+). Poly(ADP-ribose) has an N-terminal DNA-binding domain containing two zinc-fingers, which is linked to the C-terminal NAD+-binding domain by a short region containing several glutamic acid residues that are sites of auto-poly (ADP-ribosyl) ation. Production of poly(ADP-ribose) within the cell is initiated by agents that generate DNA strand interruptions. The branched homopolymer chains may reach a length of 200–300 residues but are rapidly degraded after synthesis. The function of poly(ADP-ribose) synthesis is not clear, although it seems to be required for DNA repair.

Corresponding to human Poly ADP-ribose chain.

Detect PARP using this mouse monoclonal antibody, Anti-Poly ADP-ribose Antibody, clone 10H validated for use in western blotting, IP & Immunofluorescence.

Immunoprecipitation Analysis: A representative lot was used by an an independent laboratory to immunoprecipitate Poly ADP-ribose in cultured supernatents from mouse erythrocytes coated with Poly ADP-ribose (Kawamitsu, H., et al. (1984). American Chemical Society. 3771-3777).
Immunofluorescence Analysis: A representative lot was used by an an independent laboratory to detect Poly (ADP-ribose) synthesis on transfected CV-1 cells via indirect immunofluorescence (J.H. Kupper, et al. J. Biol. Chem. (1990) 265:18721).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in untreated and UV treated HeLa cells.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected UV treated HeLa cells. Little or no signal observed in untreated HeLa cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB3192

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