MABE951
Anti-Histone H3.3 (H3-3A,H3-3B) Antibody
rat monoclonal, 6C4A3
Synonym(s):
Histone H3.3, H3.3A, H3F3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
6C4A3, monoclonal
Species reactivity:
human, monkey, mouse, canine
Application:
WB
Citations:
3
Product Name
Anti-Histone H3.3 Antibody, clone 6C4A3, clone 6C4A3, 1 mg/mL, from rat
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6C4A3, monoclonal
species reactivity
human, monkey, mouse, canine
concentration
1 mg/mL
technique(s)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H3F3B(3021)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa observed
Application
Anti-Histone H3.3 Antibody, clone 6C4A3 is an antibody against Histone H3.3 for use in western blotting.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Histone H3.3 with recombinant H3.3 proteins. Additionally, 1 µg/mL from a representative lot detected Histone H3.3 in HeLa, COS1, NIH/3T3, NRK, and MDCK whole cell lysates (Prof. Taro Tachibana, Cell Engineering Corporation.).
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Akihito Harada et al.
The EMBO journal, 31(13), 2994-3007 (2012-05-10)
Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of
Zhexin Zhu et al.
Cell stem cell, 20(2), 274-289 (2016-12-13)
The chromatin landscape and cellular metabolism both contribute to cell fate determination, but their interplay remains poorly understood. Using genome-wide siRNA screening, we have identified prohibitin (PHB) as an essential factor in self-renewal of human embryonic stem cells (hESCs). Mechanistically, PHB
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABE951 | 04053252970603 |