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NE1019

Anti-Myelin Basic Protein Mouse mAb (SMI-99)

liquid, clone SMI-99, Calbiochem®

Synonym(s):

Myelin basic protein antibody

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
SMI-99, monoclonal
Species reactivity:
guinea pig (weakly), mammals
Application:
—
Citations:
9
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

SMI-99, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

guinea pig (weakly), mammals

should not react with

chicken, porcine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000))

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MBP(4155)

General description

Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Immunogen

Epitope: Within amino acids 131-136
human myelin basic protein

Application


ELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)

Other Notes

Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197.
Shin, R.W., et al. 1991. Lab. Invest.64, 693.
Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jane L Zhu et al.
Journal of translational medicine, 20(1), 41-41 (2022-01-26)
Morphea is an autoimmune, sclerosing skin disorder. Despite the recent emphasis on immune dysregulation in morphea, the role of autoantibodies in morphea pathogenesis or utility as biomarkers are poorly defined. Autoantigen microarray was used to profile autoantibodies from the serum
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Taasin Srivastava et al.
The Journal of clinical investigation, 128(5), 2025-2041 (2018-04-18)
Cerebral white matter injury (WMI) persistently disrupts myelin regeneration by oligodendrocyte progenitor cells (OPCs). We identified a specific bioactive hyaluronan fragment (bHAf) that downregulates myelin gene expression and chronically blocks OPC maturation and myelination via a tolerance-like mechanism that dysregulates
View All Related Papers

Global Trade Item Number

SKUGTIN
NE1019-100UL04055977209884