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SCC007

ReNcell CX Human Neural Progenitor Cell Line

ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

Synonym(s):

Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells

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About This Item

UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
human
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biological source

human

Quality Level

100

manufacturer/tradename

Chemicon®, ReNcell

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

Product Source: Human cortical brain tissue
ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(Published in Nature)

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Neural Stem Cells

Preparation Note

When stored at the recommended storage conditions (liquid nitrogen), ReNcell CX cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

Analysis Note

Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Other Notes

> 1x106 viable ReNcell CX cells
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

Regulatory Information

高风险级别生物产品-Merck

This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Alzheimer’s In A Dish™: 3D Neural Stem Cell Models of Alzheimer's Disease

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Articles

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

View All Articles

Related Content

ReNcell®Human Neural Progenitors: Renewable and Consistent Supply of Human Functional Neurons

ReNcell® VM and ReNcell® CX are two well-established neural stem cell lines derived from developing human brains. ReNcell® VM and CX cells are generated from the ventral mesencephalon and cortical regions of the brain, respectively, and transduced with the myc transcription factor. Both cell lines offer phenotype and genotype stability, in addition to the multipotential neuronal differentiation capacity, over long-term culture. This article describes the characteristics and differentiation of ReNcell® lines. Results of calcium and membrane potential changes in response to various ligands are also shown. The convenience to maintain in culture and flexibility to differentiate to individual scientists’ needs make ReNcell® lines the ideal platform for research and discovery.

ReNcell™ CX Human Neural Progenitor Cell Line
ReNcell VM Cell Line - A Model of Human Neural Development and Differentiation
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International journal of environmental research and public health, 17(20) (2020-10-15)
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by disruptions in social communication and behavioral flexibility. Both genetic and environmental factors contribute to ASD risk. Epidemiologic studies indicate that roadway vehicle exhaust and in utero exposure to diesel particulate
Jung-Hwa Oh et al.
International journal of molecular medicine, 41(2), 783-790 (2017-12-06)
Alternative cell models of human neural stem cells (hNSCs) have been developed and used for investigations ranging from in vitro experiments to in vivo clinical studies. However, a cell model capable of mimicking the 'normal' state of hNSCs is mandatory in order
Qi Gu et al.
Methods in molecular biology (Clifton, N.J.), 1758, 129-138 (2018-04-22)
Bioprinting provides an opportunity to produce three-dimensional (3D) tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a method for fabricating human neural tissue by 3D printing human neural stem cells with a bioink
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Global Trade Item Number

SKUGTIN
SCC00704053252358609