Key Documents

View All Documentation

SCR530

STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Name: STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
Brand: MM

The STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of mouse & human induced pluripotent stem cells.

Select a Size

Change View

About This Item

UNSPSC Code:

12352207

NACRES:

NA.81

eCl@ss:

32161000

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Quality Level

100

manufacturer/tradename

STEMCCA

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

shipped in

dry ice

storage temp.

-65 to -96°C

Description

General description

The STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs), respectively. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

Analysis Note

Tested to confirm the generation of iPS cells from p3 mouse embryonic fibroblasts and p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

Other Notes

1. EF1α-STEMCCA (OKSM) Lentivirus: (Part number CS204279) Three (3) vials containing 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.

Still not finding the right product?

Explore all of our products under STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

诱导多能干细胞重编程实验方案

产生人诱导多能干细胞（iPSC）的干细胞重编程实验方案，包括基于病毒和非病毒RNA的方法。

Induced Pluripotent Stem Cell Reprogramming Protocols

Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.

Global Trade Item Number

SKU	GTIN
SCR530	04053252024481