UFC5030
Amicon® Ultra Centrifugal Filter, 30 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 30 kDa
Synonym(s):
Centrifugal concentrator, 30 kDa MWCO, 0.5 mL sample volume
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About This Item
Quality Level
material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
30 kDa MWCO
greener alternative category
shipped in
ambient
General description
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application
- Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Other Notes
Legal Information
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Storage Class
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
连续洗滤法清除蛋白或核酸样品中的去垢剂，即在过滤的同时连续补加不含去垢剂的缓冲液，保持样品体积恒定。
如要从少量(0.5-10 mL)样品中纯化抗体，推荐使用Amicon® Pro纯化系统。
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
Articles
纳米颗粒纯化和生产中过滤方法和滤膜的选择。
本页内容讨论了使用超滤膜进行蛋白样品制备的原理和优势。应用包括溶剂置换、蛋白样品浓缩或纯化。
Amicon Pro 让蛋白纯化更简单。此系统可提供一致的样品制备，可实现更可靠的蛋白回收率、同时保证纯度、简化数据生成。
Related Content
浓缩你的蛋白质，别失去它。使用Amicon® Ultra过滤器进行浓缩，能可靠回收蛋白质。
Global Trade Item Number
| SKU | GTIN |
|---|---|
| UFC503024 | 08436037125867 |
| UFC503096 | 04053252464591 |
| UFC503008 | 04053252576331 |
| UFC5030BK | 04053252664656 |