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10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

Synonym(s):

inorganic pyrophosphatase

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About This Item

UNSPSC Code:
12352204
EC Number:
3.6.1.1 (BRENDA, IUBMB)
Specific activity:
~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)
Biological source:
yeast
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biological source

yeast

form

suspension

specific activity

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

packaging

pkg of 1 mL (1 mg)

manufacturer/tradename

Roche

storage temp.

2-8°C

General description

Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

Application

Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification
Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.

Biochem/physiol Actions

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.
Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Analysis Note

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number
14156423
14156422
13529723
13529725
14156429

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Tracking the m7G-cap during translation initiation by crosslinking methods.
Gross L, et al.
Methods, 137, 3-10 (2018)
2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO.
Picard-Jean F, et al.
PLoS ONE, 13(3), e0193804-e0193804 (2018)
Yakov Y Woldman et al.
Free radical biology & medicine, 47(10), 1339-1345 (2009-09-16)
Nitric oxide (NO) is a free radical involved in many physiological processes including regulation of blood pressure, immune response, and neurotransmission. However, the measurement of extremely low, in some cases subnanomolar, physiological concentrations of nitric oxide presents an analytical challenge.
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Global Trade Item Number

SKUGTIN
1010898700104061838666970