Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
PubChem Substance ID:
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
3675235
MDL number:
NACRES:
NA.54
assay
>99% (titration)
form
solid
packaging
pkg of 500 g
manufacturer/tradename
Roche
technique(s)
FISH: suitable
pH
7.0-9.0
useful pH range
7.0-9.0
pKa (25 °C)
8.1
absorption
<0.02 at 300 nm at 100 mg/mL
storage temp.
room temp
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
Application
Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer.
Features and Benefits
Crystals
Analysis Note
Purity: >99% Tris-HCl (titrimetric)
Heavy metals: <1 ppm Pb and Fe
Heavy metals: <1 ppm Pb and Fe
Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Explore all of our products under Tris hydrochloride
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
MNase Digestion for Nucleosome Mapping in Neurospora.
Cigdem S
Bio-protocol null
Izabela Jęśkowiak et al.
International journal of molecular sciences, 22(20) (2021-10-24)
(1) Background: Melanoma is an aggressive neoplasm derived from melanocyte precursors with a high metastatic potential. Responses to chemotherapy and immunotherapy for melanoma remain weak, underlining the urgent need to develop new therapeutic strategies for the treatment of melanoma. (2)
Sen Wu et al.
Nature protocols, 3(6), 1056-1076 (2008-06-13)
We describe here a streamlined procedure for targeting vector construction, which often is a limiting factor for gene targeting (knockout) technology. This procedure combines various highly efficient recombination-based cloning methods in bacteria, consisting of three steps. First step is the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10812846001 | 04061838678157 |