Skip to Content
Merck
CN

Key Documents

View All Documentation

10843555001

Roche

Hygromycin B

from Streptomyces hygroscopicus

Synonym(s):

Hygromycin B from Streptomyces hygroscopicus

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
31282-04-9
Molecular Weight:
527.52
UNSPSC Code:
12352200
PubChem Substance ID:
329749191
MDL number:
MFCD06795479
NACRES:
NA.54
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Streptomyces hygroscopicus

sterility

non-sterile; 0.2 μm filtered

assay

80% (HPLC)

form

buffered aqueous solution

composition

Hygromycin B, >80% HPLC

packaging

pkg of 20 mL (1 g)

manufacturer/tradename

Roche

concentration

50 mg/mL

technique(s)

transfection: suitable

SMILES string

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

General description

  • Formula: C20H37N3O13
  • LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
  • Molecular weight: Mr = 527.5
  • Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.

Application

Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Physical form

Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane

Preparation Note

Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.
Store at 2 to 8 °C. (solution)

Analysis Note

Purity: >80% (HPLC)

Other Notes

For life science research only. Not for use in diagnostic procedures.
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.
Unit Assay: Unit/mg
We do not have this information.

Still not finding the right product?

Explore all of our products under Hygromycin B

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
21414000
14736900
11554000
10788700
10172700

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

抗生素杀灭曲线

抗生素杀灭曲线是一项剂量响应实验，通过逐渐增大施加在哺乳动物细胞中的备选抗生素剂量进行测定。

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

Yiping Qi et al.
Methods in molecular biology (Clifton, N.J.), 712, 21-30 (2011-03-02)
Plant disease resistance (R) proteins confer strong resistance against pathogens by recognizing particular pathogen effectors. Identification of proteins associated with an R protein will provide insight into the mechanism of R protein function. Many R proteins are associated with the
Wouter Koole et al.
Nucleic acids research, 41(16), e158-e158 (2013-07-19)
Here, we report the investigation of microsatellite instability (MSI) in human cells with a newly developed reporter system based on fluorescence. We composed a vector into which microsatellites of different lengths and nucleotide composition can be introduced between a functional
Elisa Gobbini et al.
Cell reports, 33(3), 108287-108287 (2020-10-22)
Homologous recombination is initiated by nucleolytic degradation (resection) of DNA double-strand breaks (DSBs). DSB resection is a two-step process in which an initial short-range step is catalyzed by the Mre11-Rad50-Xrs2 (MRX) complex and limited to the vicinity of the DSB
View All Related Papers

Global Trade Item Number

SKUGTIN
1084355500104061838251831