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About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
form
liquid
packaging
pkg of 1 mL
manufacturer/tradename
Roche
storage temp.
-10 to -25°C
General description
Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.
Application
Streptavidin-POD has been used for:
- ELISA
- In situ hybridization
- Western blot
Physical form
Liquid, stabilized
Preparation Note
Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
- ELISA: 50 to 200 mU/ml
- Immunohistocytochemistry: 250 to 1000 mU/ml
- Western blot: 200 to 500 mU/ml
Other Notes
For life science research only. Not for use in diagnostic procedures.
One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11089153001 | 04061838250643 |