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About This Item
UNSPSC Code:
12352207
NACRES:
NA.54
Biological source:
rat tail
Form:
lyophilized (clear, colorless solution after reconstitution)
Technique(s):
cell culture | mammalian: suitable
biological source
rat tail
sterility
sterile
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 30 mg
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
rat ... Col1a1(29393)
General description
Collagens are abundantly present in vertebrates. 41 specific genes codes for 42 polypeptide chains and produce 27 collagen types. Collagens are mainly present in the extracellular matrix. Collagen type I is abundantly present in mammals. Type IV collagen is an important structural protein of basement membranes. It is chemically and genetically different from stroma collagen types I and III and cartilage collagen type II.
Application
Collagen has been used in the preparation of coverslip coating solution and collagen gel contraction assay.
Collagen is used as a substrate for culturing a variety of cells, such as coating of culture dishes and the preparation of collagen gels.
Biochem/physiol Actions
Collagen provides extracellular support for multicellular animals. Collagen type I offers mechanical stability, strength and toughness to a range of tissues from tendons and ligaments, to skin, cornea, bone and dentin.
Active on most mammalian cells.
Features and Benefits
Biological activity: Tested for the promotion of adherence of HUV-EC cells.
Contents
Lyophilizate, cell culture grade, 3 vials of 10mg
Lyophilizate, cell culture grade, 3 vials of 10mg
Preparation Note
Working concentration: 5 μg/cm2
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
IFor best results, dissolve the lyophilizate in 0.2% acetic acid (v/v) solution.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Regulatory Information
低风险生物材料
动植物源性产品
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 11179179001 | 04061838258977 |