Anti-Digoxigenin

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.

Contents: Lyophilizate

Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:

• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Western blot
• Immunofluorescence staining
• FISH (fluorescent in situ hybridization)

In the presence of Na⁺, Mg²⁺ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.

The monoclonal antibody reacts with free and bound digoxigenin.

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:

• ELISA: for coating: 2 to 4 μg/ml
• Immunohistocytochemistry: 0.5 to 2 μg/ml
• In situ hybridization: 0.2 to 0.4 μg/ml
• Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications
phosphate buffered saline, pH 7.4

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.

There are no cross reactivities known.

For life science research only. Not for use in diagnostic procedures.