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NAD100-RO

Roche

NAD

Grade I, free acid

Synonym(s):

β-Nicotinamide adenine dinucleotide hydrate, β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucleotide, NAD, Nadide

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About This Item

Empirical Formula (Hill Notation):
C21H27N7O14P2 · xH2O
CAS Number:
53-84-9
Molecular Weight:
663.43 (anhydrous basis)
UNSPSC Code:
41106305
PubChem Substance ID:
329818699
MDL number:
MFCD00036253
NACRES:
NA.54
eCl@ss:
39200202
Assay:
100% (dry weight)
Form:
lyophilized
Storage temp.:
2-8°C
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assay

100% (dry weight)

form

lyophilized

mol wt

Mr 663.4

packaging

pkg of 1 g (10127965001), pkg of 5 g (10127973001)

manufacturer/tradename

Roche

color

off-white

pH

~3.0 (50 mg/mL in water)

λmax

260 nm

shipped in

wet ice

storage temp.

2-8°C

SMILES string

NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O

InChI

1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1

InChI key

BAWFJGJZGIEFAR-NNYOXOHSSA-N

General description

Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mix for the determination of kinetic characteristics using an enzyme-linked kinetic assay.
NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.

Biochem/physiol Actions

NAD is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.
Electron acceptor
The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.

Features and Benefits

Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)

Packaging

Packaged by solid weight.

Preparation Note

Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C

Analysis Note

Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02
Absence of contaminants: LDH inhibitors are not detectable
Organic solvents: =0.05% (GC)

Other Notes

For life science research only. Not for use in diagnostic procedures.
This is the common form of NAD.

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Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
14671248
14671247
14006785
14006770
14006756

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SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation
Sarikhani M, et al.
eLife, 7(1), e32952-e32952 (2018)
Jinrui Zhang et al.
Scientific reports, 5, 12846-12846 (2015-08-06)
Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofactors. NADP(H) are critical components
The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction
Fernandes PM, et al.
The Biochemical Journal, 476(2), 179-191 (2019)
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Global Trade Item Number

SKUGTIN
1012797300104061838251756
1012796500104061838667588