10800BC
ColiComplete Kit
suitable for PCR testing in Food and Beverages applications
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About This Item
UNSPSC Code:
41116129
NACRES:
NA.85
Agency:
AOAC® 992.30 (OMA)
Suitability:
coliforms: Escherichia coli
Manufacturer/tradename:
BioControl Systems 10800-100, BioControl Systems 10800-3K
Application(s):
food and beverages
pathogen testing
pathogen testing
Storage temp.:
2-8°C
Product Name
ColiComplete, BioControl, For detection Total Coliforms and E. coli in food products
material
paper disks
agency
AOAC® 992.30 (OMA)
shelf life
6‑18 mo.
packaging
pkg of 100, pkg of 3000
manufacturer/tradename
BioControl Systems 10800-100, BioControl Systems 10800-3K
application(s)
food and beverages
pathogen testing
storage temp.
2-8°C
suitability
coliforms: Escherichia coli
General description
ColiComplete is a patented substrate supporting disc method containing both XGAL and MUG. It is designed to simultaneously detect Total Coliforms and E. coli in food products. The β-galactosidase enzyme produced by coliforms reacts with the chromogenic XGAL substrate to produce a visually detectable insoluble blue dye. The β-glucuronidase enzyme found in E. coli reacts with the MUG substrate to produce a blue fluorescent reaction under long wave UV light.
containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-β-D-glucuronide (MUG)
Application
AOAC Official Method 992.30
X-Gal, MUG
Biochem/physiol Actions
ColiComplete contains 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal) and 4-methyl umbelliferyl-β-D-glucuronide (MUG). Discs are added to LST inoculated with selected dilutions of samples. Samples are incubated at 35 - 37 oC and examined after 24 and 48 ± 2 h for confirmed total coliforms and after 30 ± 2 h for confirmed E. coli results. β-Galactosidase, from coliforms present in samples, cleaves X-Gal into 5-bromo-4-chloro-indoxyl intermediate which undergoes oxidation to yield water-insoluble blue dimer, visually detectable on disc or in surrounding medium as confirmed positive result for total coliform activity. β-Glucuronidase, from E. coli present in samples, cleaves MUG into glucuronide and methyl umbelliferone, which fluoresces under long wave UV light (366 nm) as confirmed positive result for E. coli presence.
NOTE: As E. coli O157:H7 does not produce β-glucuronidase, ColiComplete is not suitable for the detection of E. coli O157:H7.
NOTE: As E. coli O157:H7 does not produce β-glucuronidase, ColiComplete is not suitable for the detection of E. coli O157:H7.
Features and Benefits
- ColiComplete provides confirmed quantitative E. coli results in as few as 28 hours and confirmed total Coliforms result in 48 hours.
- Just add the patented ColiComplete disc to Lauryl Sulfate Broth (LST) tubes at the time of inoculation, incubate and read results. Use the Most Probably Number (MPN) format for quantitative results. No transfers are necessary.
- Simultaneous detection of both E. coli and total Coliforms from a single test. Results are confirmed and require no subculturing.
Other Notes
Bottle of reagent discs
Legal Information
AOAC is a registered trademark of AOAC International
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 10800-3K | 04061835537228 |
| 10800-100 | 04061835537211 |