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158550

Acridine Orange hemi(zinc chloride) salt

Dye content 85 %

Synonym(s):

3,6-Bis(dimethylamino)acridine hydrochloride zinc chloride double salt, 3,6-Bis(dimethylamino)acridinium chloride hemi(zinc chloride salt), Basic Orange 14

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About This Item

Linear Formula:
C17H20ClN3 · HCl · 1/2ZnCl2
CAS Number:
10127-02-3
Molecular Weight:
369.96
EC Number:
233-353-6
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24849765
MDL number:
MFCD00081043
Colour Index Number:
46005
Beilstein/REAXYS Number:
3734978
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form

powder

Quality Level

200

composition

Dye content, 85%

solubility

H2O: 1 mg/mL, clear, orange to very deep orange

λmax

489 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].Cl[H].Cl[Zn]Cl.CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C.CN(C)c4ccc5cc6ccc(cc6nc5c4)N(C)C

InChI

1S/2C17H19N3.4ClH.Zn/c2*1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;;/h2*5-11H,1-4H3;4*1H;/q;;;;;;+2/p-2

InChI key

RAHGLSRJKRXOSY-UHFFFAOYSA-L

Application

Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs).
Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Biochem/physiol Actions

Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test.

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pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H341

Hazard Classifications

Muta. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN3893
SHBM8517
SHBL9733
MKBZ7598V
MKBV0515V

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Related Content

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Differential staining of apoptotic nuclei in living cells: application to macronuclear elimination in Tetrahymena.
Mpoke SS and Wolfe J.
The Journal of Histochemistry and Cytochemistry, 45(5), 675-683 (1997)
Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system.
Traganos F
The Journal of Histochemistry and Cytochemistry, 25(1), 46-56 (1977)
The use of thionin and acridine orange in staining semithin sections of plant material embedded in epoxy resin.
R N Paul
Stain technology, 55(3), 195-196 (1980-05-01)
View All Related Papers

Global Trade Item Number

SKUGTIN
158550-25G04061838744043
158550-100G04061832338675
158550-10G04061838744036