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28514

Methylene Blue hydrate

≥95% (calc. to the dried substance)

Synonym(s):

Basic Blue 9 monohydrate ; Methylene Blue (hydrate)

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
122965-43-9
Molecular Weight:
319.85 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329753217
EC Number:
200-515-2
Beilstein/REAXYS Number:
3599847
MDL number:
MFCD00150006
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Quality Level

200

assay

≥95% (calc. to the dried substance)

form

powder

impurities

≤1% insoluble in ethanol

loss

≤22% loss on drying, 105 °C

color

very dark green

solubility

ethanol: insoluble ≤1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

General description

Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.

Application

Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:
  • in the preparation of methylene blue (MB) solution
  • to stain zymograms -to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF5228
BCCD4636
BCCC6044
BCCB2720
BCBZ7634

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Paul J Derry et al.
Nanoscale, 11(22), 10791-10807 (2019-05-28)
Previously, our group reported on the promising efficacy of poly(ethylene glycol)-hydrophilic carbon clusters (PEG-HCCs) to work as broadly active and high capacity antioxidants in brain ischemia and injury models including stroke and traumatic brain injury coupled with hemorrhagic shock. PEG-HCCs
Wei Guo et al.
Acta pharmacologica Sinica, 41(3), 423-431 (2019-06-15)
Indoleamine 2,3-dioxygenase 1 (IDO1) is emerging as a promising therapeutic target for the treatment of malignant tumors characterized by dysregulated tryptophan metabolism. However, the antitumor efficacy of existing small-molecule IDO1 inhibitors is still unsatisfactory, and the underlying mechanism remains largely

Global Trade Item Number

SKUGTIN
28514-100G04061826259559
28514-1KG04061826714058
28514-500G04061833646274