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Merck
CN

32750

3,3′-Diaminobenzidine tetrahydrochloride hydrate

for spectrophotometric det. of Se, ≥97.5%

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About This Item

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
NACRES:
NA.21
PubChem Substance ID:
UNSPSC Code:
41116105
EC Number:
231-018-9
MDL number:
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Quality Level

assay

≥97.5%

quality

for spectrophotometric det. of Se

technique(s)

UV/Vis spectroscopy: suitable

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

General description

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) is toxic in nature, and is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels.

Application

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex.

Other Notes

Reagent for the spectrophotometric determination of selenium; Peroxidase stain. Differentiation between two classes of mycobacterial catalase in polyacrylamide electrophoresis gels; Specific indication of hemoproteins in polyacrylamide gels by double staining


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

危险化学品

This item has



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HistoGreen: a new alternative to 3, 3'-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry"
Thomas MA and Lemmer B
Brain Research, 14 (2), 107-118 (2005)
Interleukin-8 in the Human Endometrium 1.
Ariciri A
J. Clin. Endocr., 83 (5), 1783-1787 (1998)
Danzuka, T. and Ueno, K.
Analytical Chemistry, 30, 1370-1370 (1958)



Global Trade Item Number

SKUGTIN
32750-1G-F04061826716908
32750-25G-F04061826716915
32750-5G-F04061826716922