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335940

Xylene Cyanol FF

Dye content ≥75 %

Synonym(s):

Acid blue 147, Cyanol FF, XC, Xylene Cyanole FF

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About This Item

Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
PubChem Substance ID:
24860353
UNSPSC Code:
12171500
Colour Index Number:
42135
NACRES:
NA.47
EC Number:
220-167-5
MDL number:
MFCD00040657
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assay

≥96.0% (HPLC)

Quality Level

200

form

powder or crystals

composition

Dye content, ≥75%

mp

295 °C (dec.) (lit.)

solubility

water: 1 mg/mL, blue

λmax

615 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=C\C(=N/CC)C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23+;

InChI key

VVLFAAMTGMGYBS-AHRQKMASSA-M

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCM8423
MKCL9865
MKCD2604
MKBT8755V
MKBK3343V

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Frequency of the BRCA1 185delAG mutation among Jewish women with ovarian cancer and matched population controls.
Muto MG
Cancer Research, 56(6), 1250-1252 (1996)
Kumar A and Garg N
Genetic Engineering (2005)
Discriminatory power of agarose gel electrophoresis in DNA fragments analysis.
Lee SV and Abdul RB et al.
Gel Electrophoresis of Nucleic Acids: A Practical Approach (2012)
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Global Trade Item Number

SKUGTIN
335940-50G04061826742730
335940-10G04061835345694