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Merck
CN

50553

α-Tri-Calcium phosphate

Reagent for transient & stable DNA transfections

Synonym(s):

α-Tricalcium phosphate, α-TCP

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About This Item

Linear Formula:
Ca3(PO4)2
CAS Number:
Molecular Weight:
310.18
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106502
EC Number:
231-840-8
MDL number:
Form:
powder
Grade:
Molecular Biology
Technique(s):
transfection: suitable
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grade

Molecular Biology

Quality Level

form

powder

technique(s)

transfection: suitable

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

storage temp.

room temp

SMILES string

[Ca++].[Ca++].[Ca++].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O

InChI

1S/3Ca.2H3O4P/c;;;2*1-5(2,3)4/h;;;2*(H3,1,2,3,4)/q3*+2;;/p-6

InChI key

QORWJWZARLRLPR-UHFFFAOYSA-H

General description

α-tri-Calcium phosphate (α-TCP) can be made by heating the low-temperature polymorph β-TCP. It can also be made by thermal crystallization of amorphous precursors with the appropriate composition above the transformation temperature. α-TCP is biocompatible as β-TCP. It is highly soluble and hydrolyses quickly to calcium-deficient hydroxyapatite. This makes α-TCP a beneficial constituent for formulating self-setting osteotransductive bone cements and biodegradable bioceramics and composites for bone repairing.
α-tri-Calcium is a tasteless powder containing excess of calcium oxide. It is commonly used to introduce DNA and transfect eukaryotic cells for molecular biology experiments.

Application

For analysis of bone cement; as standard.
α-tri-Calcium phosphate has been used to transfect 293T and 231 cells with the PiggyBac LSS-mOrange- aspartate-glutamate-valine-aspartate (DEVD)-mKate2 transposon vector and in reporter verification studies.
Suitable for
  • transient and stable transfection of wide range of cell types
  • drug delivery systems


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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A Caspase-3 Reporter for Fluorescence Lifetime Imaging of Single-Cell Apoptosis
Buschhaus J, et al.
Cells, 7(6), 57-57 (2018)
alpha-Tricalcium phosphate: Synthesis, properties and biomedical applications
Carrodeguas R G and De A S
Acta Biomaterialia, 7(10), 3536-3546 (2011)
Timo Brandenburger et al.
Shock (Augusta, Ga.), 42(3), 234-238 (2014-07-01)
Remote ischemic preconditioning (RIPC) is an easily applicable method for protecting the heart against a subsequent ischemia and reperfusion (I/R) injury. However, the exact molecular mechanisms underlying RIPC are unknown. We examined the involvement of microRNAs (miRNAs) and in particular



Global Trade Item Number

SKUGTIN
50553-10G-F04061833389881
50553-1G-F04061832433585