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67309

Kovac′s reagent for indoles

Name: Kovac′s reagent for indoles
Brand: SIAL

suitable for microbiology

Synonym(s):

4-(Dimethylamino)benzaldehyde solution

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About This Item

Empirical Formula (Hill Notation):

C₉H₁₁NO

CAS Number:

100-10-7

Molecular Weight:

149.19

UNSPSC Code:

41171621

NACRES:

NA.85

PubChem Substance ID:

329760541

MDL number:

MFCD00003381

Beilstein/REAXYS Number:

4132845

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Properties

agency

according to ISO 16654:2001

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

4-(dimethlyamino)benzaldehyde, 50 g/L , hydrochloric acid, 240 g/L , isoamylic alcohol, 710 g/L

technique(s)

microbe id | specific enzyme detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

storage temp.

2-8°C

suitability

Escherichia coli, coliforms

SMILES string

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3

InChI key

BGNGWHSBYQYVRX-UHFFFAOYSA-N

Description

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.