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Merck
CN

83400

D-(+)-Raffinose pentahydrate

suitable for microbiology, ≥99.0%, Microbiological media component

Synonym(s):

O-α-D-Galactopyranosyl-(1-6)-α-D-glucopyranosyl β-D-fructofuranoside pentahydrate, Melitose, Melitriose, O-α-D-Galactopyranosyl-(1→6)-α-D-glucopyranosyl β-D-fructofuranoside, Melitose, Melitriose

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About This Item

Empirical Formula (Hill Notation):
C18H32O16 · 5H2O
CAS Number:
Molecular Weight:
594.51
UNSPSC Code:
41106212
NACRES:
NA.85
PubChem Substance ID:
EC Number:
208-146-9
Beilstein/REAXYS Number:
99541
MDL number:
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Quality Level

assay

≥99.0% (HPLC), ≥99.0%

form

powder

optical activity

[α]20/D +105±2°, c = 10% in H2O

ign. residue

≤0.1% (as SO4)

mp

78-80 °C

solubility

H2O: 0.1 g/mL, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

As: ≤0.1 mg/kg, Ca: ≤20 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

application(s)

microbiology

storage temp.

10-30°C

SMILES string

O.O.O.O.O.OC[C@H]1O[C@H](OC[C@H]2O[C@H](O[C@]3(CO)O[C@H](CO)[C@@H](O)[C@@H]3O)[C@H](O)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C18H32O16.5H2O/c19-1-5-8(22)11(25)13(27)16(31-5)30-3-7-9(23)12(26)14(28)17(32-7)34-18(4-21)15(29)10(24)6(2-20)33-18;;;;;/h5-17,19-29H,1-4H2;5*1H2/t5-,6-,7-,8+,9-,10-,11+,12+,13-,14-,15+,16+,17-,18+;;;;;/m1...../s1

InChI key

BITMAWRCWSHCRW-PFQJHCPISA-N

General description

Raffinose is a natural trisaccharide composed of glucose, galactose, and fructose. It is a water-soluble oligosaccharide, most commonly present in legumes but also in sugar beets and remains during the process in the molasse. It is primarily used as a carbon source in the cultivation of yeast but also has certain cell proliferative and anti-oxidative properties that can prove useful in culture media. Raffinose is used by beneficial probiotic bacteria and an essential component of certain cryoprotectant solutions.

Application

D-(+)-Raffinose pentahydrate can be used as a carbon source for the cultivation of yeast
It is used by beneficial probiotic bacteria like Bifidobacteria and Lactobacilli but could be used as well for differentiation and identification of Escherichia coli, Enterococcus faecium and Streptococcus pneumoniae.
It can also be used in certain cryoprotectant solutions.



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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品

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Articles

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.


Poonam Bheda et al.
STAR protocols, 1(3), 100228-100228 (2020-12-31)
The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we
Antonio Marsella et al.
Cell reports, 34(13), 108906-108906 (2021-04-01)
The Mre11-Rad50-Xrs2 (MRX) complex detects and processes DNA double-strand breaks (DSBs). Its DNA binding and processing activities are regulated by transitions between an ATP-bound state and a post-hydrolysis cutting state that is nucleolytically active. Mre11 endonuclease activity is stimulated by



Global Trade Item Number

SKUGTIN
83400-100G04061833005668
83400-25G04061835571901