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Merck
CN

X100

Triton X-100

laboratory grade

Synonym(s):

t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

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About This Item

Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
UNSPSC Code:
12161900
NACRES:
NA.21
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
2315025
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biological source

synthetic

Quality Level

grade

laboratory grade

description

non-ionic

form

liquid

mol wt

micellar avg mol wt 80,000, average mol wt 625

aggregation number

100-155

CMC

0.2-0.9 mM (20-25°C)

mp

6  °C (43 °F)

transition temp

cloud point 65 °C, pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

General description

Triton X-100 has hemolytic property and is used for DNA extraction.
Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

  • Ideal for Cell Biology and Biochemical research applications
  • Non-ionic nondenaturing detergent
  • Superior wetting agent and emulsifier
  • Highly versatile surfactant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow


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signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter, type N95 (US)

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2



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Protocols

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

View All Protocols

Articles

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.


Growth and hydration of Triton X-100 micelles in monovalent alkali salts: a light scattering study
Molina-Bolivar JA, et al.
The Journal of Physical Chemistry B, 106(4), 870-877 (2002)
Knockdown of miR-210 decreases hypoxic glioma stem cells stemness and radioresistance
Yang W, et al.
Experimental Cell Research, 326(1), 22-35 (2003)
alpha -Synuclein Is Degraded by Both Autophagy and the Proteasome
Webb LJ, et al.
The Journal of Biological Chemistry, 278(27), 25009-25013 (2003)



Global Trade Item Number

SKUGTIN
82020-U04061833520697
82023-U04061833520581
82011-U04061838551191
82014-U04061838551214
82017-U04061833408643
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82028-U04061833408612
82032-U04061833520628
82039-U04061833508633
82042-U04061833508657
82035-U04061833520659
X100-100ML04061837603044
X100-1L04061837603068
X100-2ML04061837603075
X100-1GA04061837603051
X100-500ML04061837603082
X100-5GA04061837603099
X100-5ML04061837603105
X100-6X500ML04061837603112
HAM79433-1EA04061838551757