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A92775

Arsenazo III

calcium-sensitive dye, Powder

Synonym(s):

2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid, 2,7-Bis(2-arsonophenylazo)chromotropic acid

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About This Item

Linear Formula:
(HO)2C10H2(SO3H)2(N=NC6H4AsO3H2)2
CAS Number:
1668-00-4
Molecular Weight:
776.37
NACRES:
NA.47
PubChem Substance ID:
24891431
UNSPSC Code:
12171500
EC Number:
216-788-6
MDL number:
MFCD00036695
Beilstein/REAXYS Number:
5717957
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Product Name

Arsenazo III, calcium-sensitive dye

Quality Level

200

form

powder

technique(s)

titration: suitable

impurities

<0.1% Ca

color

brown to very dark brown, and Black

mp

>320 °C (lit.)

solubility

ammonium hydroxide: 1 mg/mL

λmax

530 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(\N=N\c2ccccc2[As](O)(O)=O)c(cc3cc(c(\N=N\c4ccccc4[As](O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H18As2N4O14S2/c29-21-18-11(9-16(43(37,38)39)19(21)27-25-14-7-3-1-5-12(14)23(31,32)33)10-17(44(40,41)42)20(22(18)30)28-26-15-8-4-2-6-13(15)24(34,35)36/h1-10,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

UQHVTNUJRKELCE-NBHCHVEOSA-N

Application

Arsenazo III has been used as a color forming reagent to determine uranium concentration.

Biochem/physiol Actions

Arsenazo III is a metallochromic dye. Arsenazo III is used in the determination of calcium in biological samples. It is used to evaluate calcium transport in permeabilized cells. It is also used for the detection of rare earth metals (polyvalent metal ions). Arsenazo III is used for the spectrophotometric determination of uranium and thorium.

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pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN9453
MKCL7154
MKCG3974
MKCF4588
MKBT7566V

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Daniel T Holmes et al.
American journal of clinical pathology, 151(3), 328-336 (2018-11-27)
The Hoffmann method is a procedure for reference interval estimation using routine clinical results. Many authors incorrectly prepare Hoffmann plots on a linear rather than normal probability scale. We explore the consequences. This was investigated algebraically, by random number simulations
J W Janssen et al.
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies, 29(3), 197-201 (1991-03-01)
The widely used calcium dye reagent, o-cresolphthalein complexone, was replaced by the less well known metallochromic indicator Arsenazo III in a selective multitest analyser. Calibration stability increased from 12 hours to more than 48 hours. With the Arsenazo III reagent
Analytica Chimica Acta, 71, 375-375 (1974)
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Global Trade Item Number

SKUGTIN
A92775-10G04061833401712
A92775-1G04061833401903
A92775-5G04061832340029